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sars-cov-2 (2019-ncov) spike(b.1.351) gene orf cdna clone expression plasmid (codon optimized), c-his tag  (Sino Biological)


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    Sino Biological sars-cov-2 (2019-ncov) spike(b.1.351) gene orf cdna clone expression plasmid (codon optimized), c-his tag
    Sars Cov 2 (2019 Ncov) Spike(b.1.351) Gene Orf Cdna Clone Expression Plasmid (Codon Optimized), C His Tag, supplied by Sino Biological, used in various techniques. Bioz Stars score: 91/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/codon/SARS-CoV-2+(2019-nCoV)+Spike(B%2E1%2E351)+Gene+ORF+cDNA+clone+expression+plasmid+(Codon+Optimized)%2C+C-His+tag/custom%40vg40772-ch%4042633862
    Average 91 stars, based on 4 article reviews
    sars-cov-2 (2019-ncov) spike(b.1.351) gene orf cdna clone expression plasmid (codon optimized), c-his tag - by Bioz Stars, 2026-09
    91/100 stars

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    Related Articles

    other:

    Article Title: Nasal mucus-derived KLK13 restricts SARS-CoV-2 infection via proteolytic cleavage of spike
    Article Snippet: The plasmids pCMV3-KLK13-HA (HG10199-CY), pCMV3-PCSK4 (HG21911-UT), pCMV3-SARS-CoV-1 spike-FLAG (VG40150-CF), and pCMV3-FLAG-HKU1 spike (VG40021-NF) were purchased from SinoBiological (Beijing, China).

    Polymerase Chain Reaction:

    Article Title: Vaccine molecules
    Article Snippet: .. Briefly, HA from A/Hong Kong/483/97 (H5N1), A/northern shoveler/California/HKWF115/07 (H6N1), A/pintail duck/Alberta/114/1979 (H8N4), A/Hong Kong/1073/99 (H9N2), A/duck/Yangzhou/906/2002 (H11N2), and A/black-headed gull/Netherlands/1/00 (H13N8) were picked up by PCR from cDNA (VG11689-C; VG11723-C; VG11722-C; VG11229-C; VG11705-C, VG11721-C, all from Sino Biological Inc., Beijing, China), and cloned into SfiI-sites in the CMV-based pLNOH2 vector (27). ..

    Clone Assay:

    Article Title: Vaccine molecules
    Article Snippet: .. Briefly, HA from A/Hong Kong/483/97 (H5N1), A/northern shoveler/California/HKWF115/07 (H6N1), A/pintail duck/Alberta/114/1979 (H8N4), A/Hong Kong/1073/99 (H9N2), A/duck/Yangzhou/906/2002 (H11N2), and A/black-headed gull/Netherlands/1/00 (H13N8) were picked up by PCR from cDNA (VG11689-C; VG11723-C; VG11722-C; VG11229-C; VG11705-C, VG11721-C, all from Sino Biological Inc., Beijing, China), and cloned into SfiI-sites in the CMV-based pLNOH2 vector (27). ..

    Article Title: The SARS-CoV-2 nucleocapsid protein induces microglia senescence-mediated cognitive impairment via Glycolysis
    Article Snippet: Dulbecco’s modified Eagle’s medium (Lot: 12491015, DMEM, Gibco, USA) containing 4.5 g/L glucose was supplied with 10% fetal bovine serum (Lot: 164250, Procell) and 1% penicillin-streptomycin (Lot: PB180120 , Procell) for maintaining cells in a humid environment containing 5% CO 2 at 37 °C. .. Codon-optimized pLJM1-SARS-CoV-2 N-FLAG was cloned from SinoBiological (cat# VG40588-NF) using BamHI and EcoRI. .. Transient transfections were performed using Lipofectamine 3000 (Lot: L3000001, Invitrogen, Thermo Fisher Scientific, USA) according to the manufacturer’s guidelines.

    Plasmid Preparation:

    Article Title: Vaccine molecules
    Article Snippet: .. Briefly, HA from A/Hong Kong/483/97 (H5N1), A/northern shoveler/California/HKWF115/07 (H6N1), A/pintail duck/Alberta/114/1979 (H8N4), A/Hong Kong/1073/99 (H9N2), A/duck/Yangzhou/906/2002 (H11N2), and A/black-headed gull/Netherlands/1/00 (H13N8) were picked up by PCR from cDNA (VG11689-C; VG11723-C; VG11722-C; VG11229-C; VG11705-C, VG11721-C, all from Sino Biological Inc., Beijing, China), and cloned into SfiI-sites in the CMV-based pLNOH2 vector (27). ..

    Stable Transfection:

    Article Title: Identification of four compounds as potential inhibitory agents for SARS-CoV-2 spike protein
    Article Snippet: Cells and pseudoviruses HEK293T cells were obtained from the American Type Culture Collection (ATCC). .. The 293T/hACE2 & TMPRSS2 stable cell line, along with plasmids encoding SARS-CoV-2 Spike RBDs for various strains − 2019-nCoV (VG40592-CF), B.1.617.2 (VG40864-UT), and BA.4/BA.5/BA.5.2 (VG40908-CH) were purchased from Sino Biological (Beijing, China). .. Additionally, SARS-CoV-2 pseudo viruses for strains 2019-nCoV (PSV001-2), B.1.617.2 (PSV011-2), and Omicron BA.5 (PSV023-2) were also sourced from Sino Biological.



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